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PCR ribotype 027 specific SNP results in premature translation stop. C. difficile 630 CDS CD1761 orthologues in R20291 and CD196 contain two adjacent single nucleotide polymorphisms (SNPs); a synonymous G→A and a non-synonymous C→T which results in the introduction of an ochre stop codon (TAA) at amino acid 126. Red bars indicates ≥99% homology between DNA sequences.
