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. 2010 Nov 29;2(1):45–49. doi: 10.4161/trns.2.1.14306

Figure 2.

Figure 2

After heat shock, levels of Ser5-P substantially decrease at the actin promoter, but levels of Cdk7 and cyclin H remain relatively stable. (A) Phosphorylation of Ser5 is significantly decreased at the actin promoter after heat shock. ChIP assays were performed with antibodies against the N-terminus of Rpb1 and Ser5-P at the actin promoter, as well as the N-terminus of Rpb1 in a downstream region of the actin gene. (B) Levels of general transcription factors, including the Cdk7 and cyclin H subunits of TFIIH, do not substantially decrease at the actin promoter after heat shock. ChIP assays were performed with antibodies against the indicated factors.