Skip to main content
. 2010 Nov 29;2(1):45–49. doi: 10.4161/trns.2.1.14306

Figure 4.

Figure 4

To repress phosphorylation of the CTD by TFIIH, B2 RNA must build into complexes on promoter DNA along with Pol II. (A) The time course indicates the points at which general transcription factors, Pol II and B2 RNA (or B1 RNA) were added to reactions. B2 RNA was added either before (point 1) or after (point 2) pre-initiation complex formation. Where indicated, complexes were treated with RNase 1 (10 U, NEB) prior to adding TFIIE and TFIIH. (B) Model depicting that the presence of B2 RNA at a promoter prevents phosphorylation of Ser5 residues on the CTD by TFIIH.