Table 3.
Comparison of QRT-PCR validation and microarray mean relative values1
Genbank | Gene Symbol | Map | Microarray | QRT-PCR | Pathway |
---|---|---|---|---|---|
NM_178502 | DTX3 | 12q13.2 | 0.57 (0.22) | 0.63 (0.30) | Notch |
NM_004423 | DVL3 | 3q27 | 0.38 (0.13) | 0.53 (0.15) | WNT |
NM_030775 | WNT5B | 12p13.3 | 0.53 (0.14) | 0.15 (0.51) | WNT |
NM_006172.3 | NPPA | 1p36.22 | 2.2 (0.59) (shunt) 19.4 (5.02) (no shunt) | 2.1 (0.32) (shunt) 19.3 (0.82) (no shunt) | BMP |
1. Expression level (fold change) relative to the normalized control values set at 1, mean (+/- sd).