Western blot showing sheep-anti-mouse-IgG [polyclonal, directed
against both heavy and light chains] (A) and RCA
binding (B) to purified antibody produced in hybridoma
culture (lane 1), tobacco plants (lane 2), and two tobacco plants
(GalT-8 × Mgr-48–11 and -12) coexpressing GalT (lanes 3 and 4).
An amount of 0.17 μg hybridoma antibody was used per lane, and equal
amounts of the other antibodies as judged from the intensity of the
heavy chain bands on Coomassie-stained gels (not shown). H.C.,
heavy chain; L.C., light chain.