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The bceBΔloop pDGbceR strain was grown in medium containing IPTG (1 mM). Cells were disrupted and subjected to sub-cellular fractionation. Lane 1, crude lysate; lane 2, supernatant resulting from low speed centrifugation; lane 3, supernatant resulting from high speed centrifugation; lane 4, pellet resulting from high speed centrifugation (membrane fraction). Western blots were probed with a rabbit anti-BceA antibody.
