Table 3. Primers used for real-time RT-qPCR.
Gene | FORWARD | REVERSE | Accession No. | Product length |
PRPF3 | GGTGGGACTCGTACATCATAC | GGGTAACTGGTGTATCATTGTCA | NM_027541 | 141 bp |
PRP4 | CCTCCTCCACGACAACCAAA | CCCAAAATCCCAGACTCTCC | NM_027297 | 136 bp |
PRPF31 | GAGAGCACAGAAGGGAAGG | GGGCTTCACCTGCTTCACG | NM_027328 | 99 bp |
PRPC8 | GTGGATGCTCAGAAGGAAGACA | GAGGACTGCATGAGGCATGTA | NM_138659 | 141 bp |
U1-70K | CGGAGAGAGTTTGAGGTGTATG | GTGCTTGTAAGCGGAGTGCAT | NM_009224 | 133 bp |
U2-SF3a | GGAATTTGAGGAGCTGCTGAAG | GGTAGCAGTCATGGAGATCAAG | NM_029157 | 119 bp |
Rho | GCCACACTTGGAGGTGAAATC | CCACACCCATGATAGCGTGAT | NM_145383 | 125 bp |
NRL | CTGTGCCTTTCTGGTTCTGACA | GGACTGAGCAGAGAGAGGT | NM_008736 | 83 bp |
RP1 | GCAGGTTGCATATCCAGTGAC | GGAGAAACTAGTAGAAGGTGTGT | BC_0996607 | 123 bp |
RDS | GGAGGTCAAAGATCGCATCAAG | GGTGAGCTGGTACTGGATACA | NM_008938 | 115 bp |
H4d | CATCACCAAGCCCGCCATC | GGAACACCTTCAGCACACCA | NM_175654 | 101 bp |
Beta 2M | GGTCGCTTCAGTCGTCAGCAT | GCAGTTCAGTATGTTCGGCTTC | NM_009735 | 154 bp |
Rho:Rhodopsin; NRL: neural retina leucine zipper; RP1: retinitis pigmentosa 1; RDS: retina degeneration slow; H4d: Histone cluster 1, H4d; Beta 2M: beta 2-Microglobulin.