Table 1. Oligonucleotides used in the different real-time LCR trials.

Oligonucleotide	Sequence 5′→3′	Tm a
LCPR1 b	CACTTCCCAGCATGTAGCCAC	68.6°C
LCPR2	GTGGCTACATGCTCGGAAGTGT	69.6°C
LCPR3S	AAGAGGCCTGCTCATGA	66.7°C
LCPR4S b	CATGAGCAGGCCTCTT	65.0°C
LCPR2G	GTGGCTACATGCTCGGAAGTG	68.9°C
LCPR3G	AAGAGGCCTGCTCATG	65.0°C
LCPR3L	CAAAATGTATAAGAGGCCTGCTCATGA	69.8°C
LCPR4L b	CATGAGCAGGCCTCTTATACATTTTG	68.9°C

The nucleotides which complement to the mutant SNP target are underlined on the discriminating oligonucleotides.

^aThe melting temperature T_m was estimated using the DINAMelt web server (http://www.bioinfo.rpi.edu/applications/hybrid/hybrid2.php).

^b5′ phosphorylation on the ligating oligonucleotide.