Table 3. Experimental intra- and inter-assay variability of the basic real-time LCR protocol.

Variation	Frequency of V136	CV (%)
Intra-assay	100%	11.29
Intra-assay	25%	21.45
Intra-assay	6.25%	26.17
Intra-assay	1.56%	26.54
Intra-assay	0.39%	18.03
Inter-assay	100%	15.32
Inter-assay	25%	15.97
Inter-assay	6.25%	17.00
Inter-assay	1.56%	12.80
Inter-assay	0.39%	9.47

The coefficient of variation (CV) was calculated from the measured mutant V₁₃₆ frequencies in the plasmid standards.