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Incubation of pCRP in polystyrene flasks leads to a partial dissociation of the protein to mCRP. After various hours of incubation time samples of the incubation assays with pCRP were dot-blotted and the blots probed with mCRP (clone 9C9) or pCRP (clone 8D8) specific antibodies. Whereas the pCRP staining shows a mild decrease the mCRP staining increases gradually in the course of the experiment.
