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. 2011 Jan 19;6(1):e14547. doi: 10.1371/journal.pone.0014547

Table 1. Physical characteristics of DIA1 proteins and similarity to orthologues from key species.

BLASTPd similarity toe:
Metazoan speciesa Length protein (amino acids) pIb Molecular massc (kDa) H. sapiens G. gallus D. rerio (a) C. intestinalis S. purpuratus D. melanogaster N. vectensis
Cnidaria
Nematostella vectensis 402 5.6 44.7 8e-46 4e-44 6e-49 1e-15 6e-47 7e-08 -
Echinodermata
Strongylocentrotus purpuratus 431 7.9 49.7 6e-80 3e-81 5e-84 3e-17 - 2e-08 2e-42
Arthropoda
Hexapoda
Aedes aegypti 395 6.0 45.7 6e-16 6e-15 2e-14 1e-04 5e-16 6e-14 5e-15
Anopheles gambiae 412 5.4 47.3 2e-06 3e-08 9e-09 0.10 5e-12 4e-10 0.018
Culex pipiens 400 5.1 45.2 2e-13 4e-13 8e-16 5e-04 1e-15 2e-13 2e-18
Drosophila melanogaster 348 5.3 40.0 1e-06 7e-07 2e-08 >10 2e-08 - 5e-08
Nasonia vitripennis 404 5.1 46.2 3e-53 7e-53 2e-54 1e-11 9e-47 2e-08 4e-36
Chordata
Urochordata
Ciona intestinalis 477 9.3 54.5 2e-29 1e-31 3e-34 - 3e-17 >10 4e-14
Cephalochordata
Branchiostoma floridae 398 5.2 46.1 3e-101 5e-98 9e-104 2e-31 5e-81 7e-12 6e-43
Vertebrata
Neopterygii
Danio rerio (a) 432 8.6 50.0 0.0 0.0 - 6e-32 8e-82 2e-07 1e-46
Danio rerio (b) 429 8.7 49.4 0.0 0.0 0.0 6e-31 4e-80 2e-06 3e-44
Gasterosteus aculeatus 434 8.9 50.0 0.0 0.0 0.0 6e-33 5e-81 3e-06 2e-44
Oryzias latipes 434 8.8 50.1 0.0 0.0 0.0 2e-32 3e-83 3e-06 2e-44
Takifugu rubripes 435 8.8 50.1 0.0 0.0 0.0 3e-34 8e-84 1e-07 6e-47
Tetraodon nigroviridis 425 8.7 49.2 0.0 0.0 0.0 6e-32 6e-82 1e-05 9e-45
Tetrapoda
Gallus gallus 429 8.6 49.0 0.0 - 0.0 3e-29 4e-79 7e-06 9e-42
Xenopus tropicalis 429 8.7 49.3 0.0 0.0 0.0 7e-32 3e-80 1e-06 7e-45
Bos taurus 430 8.8 49.5 0.0 0.0 0.0 1e-29 6e-80 2e-06 3e-41
Canis familiaris 430 8.8 49.5 0.0 0.0 0.0 1e-29 6e-80 2e-06 3e-41
Homo sapiens 430 8.8 49.4 - 0.0 0.0 1e-29 7e-80 2e-06 3e-41
Macaca mulatta 430 8.8 49.5 - 0.0 0.0 1e-29 7e-80 2e-06 3e-41
Monodelphis domestica 430 8.8 49.3 0.0 0.0 0.0 9e-30 6e-80 8e-07 2e-42
Mus musculus 430 8.9 49.5 0.0 0.0 0.0 2e-30 5e-79 9e-06 8e-41
Pan troglodytes 430 8.8 49.5 0.0 0.0 0.0 1e-29 6e-80 2e-06 3e-41
Pongo pygmaeus 430 8.8 49.5 0.0 0.0 0.0 1e-29 7e-80 2e-06 3e-41
Pteropus vampyrus 430 8.9 49.5 0.0 0.0 0.0 4e-29 7e-80 2e-05 8e-41
Rattus norvegicus 430 8.9 49.5 0.0 0.0 0.0 2e-30 5e-79 9e-06 8e-41
Tursiops truncatus 430 8.8 49.5 0.0 0.0 0.0 1e-29 7e-80 2e-06 3e-41
a

DIA1 homologues were restricted to the Metazoa, but were not found in the phylum Porifera or Nematoda. Partial homologues, including those found in the phylum Mollusca and Annelida (Table S2), are not shown. See Table S1 for accession numbers of full length DIA1 proteins. Only a single Drosophila species DIA1 is included here. Table S3 is an expanded version of this Table.

b

Isoelectric point calculated using the assumption that all residues have pKa values equivalent to that of isolated residues, and so may not accurately represent the value for the folded protein.

c

Isotopically-averaged molecular weight prediction in kiloDaltons.

d

The BLASTP E-value measures the statistical significance threshold for protein sequence matches. The smaller the number, the better the match. Computer shorthand nomenclature is used to present E-values when values are small. For example, 5e-01 = 0.5 and 5e-04 = 0.0005. Values below 1e-250 are indicated as zero, and details of those greater than 10 are not provided. A dash is used when protein alignments have 100% identity.

e

Proteins were compared to DIA1from H. sapiens, C. intestinalis, S. purpuratus, or D. melanogaster, or N. vectensis by protein BLAST. The DIA1 proteins used for comparison were chosen as representives from Class Mammalia, Class Aves, and Class Neopterygii within the subphylum Vertebrata, subphylum Urochordata, phylum Echinodermata, phylum Arthropoda, and phylum Cnidaria, respectively.