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. 2011 Jan 19;6(1):e16207. doi: 10.1371/journal.pone.0016207

Figure 2. Chronic CSE treatment elevated PDK2 expression, increasing PDHα phosphorylation.

Figure 2

A, the protein levels of PDK2 were determined 4 h after switching from culture medium to Krebs-Henseleit buffer in chronic CSE treated OKF6 cells. PDK2 were overexpressed by 2.2±0.1-fold in the CSE treated OKF6 cells versus its corresponding untreated cells (n = 3, P<0.05). B. the growth of chronic CSE treated OKF6 cells were significantly inhibited after treatment with 10 and/or 20 mM dichloroacetate for 48 h. Columns, means of three individual experiments; bars, SD. DCA, dichloroacetate. *P<0.05, #P>0.05. C. western blot for PDHα and phosphorylated PDHα. Results are representative of three independent experiments. CTRL, control untreated OKF6 cells; CSE, chronic CSE treated OKF6 cells.