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. 2011 Jan 19;6(1):e16207. doi: 10.1371/journal.pone.0016207

Figure 5. Chronic CSE treatment increased ROS, increasing PDK2 expression, resulting in enhanced HIF1α expression.

Figure 5

A, ROS activity was measured in triplicate with the intensity shift from a mean of 74.2 in the control cells (light gray) to a mean of 102.6 in the CSE treated OKF6 cells (dark gary) (n = 3, P<0.05) by flow cytometry. B. In chronic CSE treated OKF6 cells, introduction of ROS scavengers, N-acetylcysteine decreased HIF1α expression. Results are representative of three independent experiments. NAC, N-acetylcysteine. C. In chronic CSE treated OKF6 cells, introduction of ROS scavenger, N-acytylcysteine, decreased PDK2 Expression. NAC, N-acetylcysteine. The experiment was repeated twice with similar results. D. HIF1α levels in chronic CSE treated OKF6 cells exposed to 5 mM dichloroacetate (DCA, PDK2 inhibitor) for 24 h. The experiments were performed twice independently with similar results. CTRL, control untreated OKF6 cells; CSE, chronic CSE treated OKF6 cells.