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. 2001 Feb 20;98(5):2905–2910. doi: 10.1073/pnas.051618398

Figure 3.

Figure 3

HPLC elution profiles and absorption spectra of carotenoids produced in a lycopene-accumulating E. coli strain (14) in the presence of cDNAs encoding a lettuce lycopene ɛ-cyclase (A) and an Arabidopsis lycopene ɛcyclase (B). The elution profile of a control culture, containing the empty cloning vector [pBluescript SK(−)], is shown in C. Absorption spectra in D–F are for the indicated peaks in the adjacent elution profiles. Absorption maxima (for the pigments in the HPLC mobile phase; see Materials and Methods) are listed for each spectrum. Elution times were ≈12.2 min for lycopene (ψ,ψ-carotene), 14.3 min for δ-carotene (ɛ,ψ-carotene), and 17.1 min for ɛ-carotene (ɛ,ɛ-carotene).