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. 2001 Feb 20;98(5):2905–2910. doi: 10.1073/pnas.051618398

Figure 5.

Figure 5

Schematic illustration of truncated and chimeric lycopene ɛ-cyclase cDNAs. Carotenoids that accumulate in an E. coli strain that contains the indicated cDNA [in plasmid vector pBluescript SK(−)], and that otherwise accumulates only lycopene (ψ,ψ-carotene), are indicated to the right. Only the predominant carotenoid (>90% of the total in all cases) is listed. Solid black vertical lines connecting the Arabidopsis and lettuce cyclases (Top) indicate identically conserved amino acid residues. LsE/AtE 323/320 defines a chimera consisting of the 5′ portion of the lettuce ɛ-cyclase cDNA up to and including nucleotide bases specifying amino acid residue 323 and the 3′ portion of the Arabidopsis ɛ-cyclase cDNA beginning with nucleotide bases that encode amino acid residue 320 and proceeding to the end of the cDNA.