Table 1.
Activity of lycopene ɛ-cyclases and site-directed mutants with lycopene (Ψ,Ψ-carotene) as substrate in E. coli
| ɛ-Cyclase cDNA | Mutation | *Carotenoids lyc ∶ del ∶ eps |
|---|---|---|
| (None) | — | 100 ∶ 0 ∶ 0 |
| Arabidopsis (AtE) | Wild type (y2) | 1 ∶ 98 ∶ 1 |
| AtE | ALIVQF447-52SHIVLM | 0 ∶ 2 ∶ 98 |
| AtE | L448H | 0 ∶ 8 ∶ 92 |
| AtE | L448R | 0 ∶ 8 ∶ 92 |
| AtE | L448D | 37 ∶ 56 ∶ 8 |
| AtE | A447D | 1 ∶ 98 ∶ 1 |
| Lettuce (LsE) | Wild type (DY4) | 3 ∶ 8 ∶ 90 |
| LsE | H457R | 3 ∶ 6 ∶ 91 |
| LsE | H457D | 22 ∶ 18 ∶ 60 |
| LsE | H457L | 17 ∶ 73 ∶ 10 |
| Adonis (AaE1) | Wild type (Ad3) | 0 ∶ 44 ∶ 56 |
*
Carotenoids accumulated in a strain of E. coli that also contains the plasmid pAC-LYC (14) and thereby normally accumulates lycopene. lyc, lycopene; del, δ-carotene; eps, ɛ-carotene. Data given as mol ∶ mol ∶ mol.