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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Dec 17;879(2):131–138. doi: 10.1016/j.jchromb.2010.11.021

Table 2. Oxygen affinity (P50) and cooperativity (n) of purified Hbs. The starting material used for each purification is indicated by the suffix “Control” and the final product is indicated by the suffix “IMAC”.

Sample P50 (mm Hg) n
S1F HbA Control 12.3 2.5
S1F HbA-IMAC 12.2 ± 0.2 2.7 ± 0.2
S3R HbA Control 12.6 2.8
S3R HbA-IMAC 13.0 ± 0.9 2.7 ± 0.4
S1F bHb Control 24.8 3.0
S1F bHb-IMAC 25.5 ± 0.7 2.8 ± 0.1
S3R bHb Control 26.4 2.7
S3R bHb IMAC 26.1 ± 0.5 2.7 ± 0.1
S1F cHb Control 28.8 2.2
S1F cHb-D IMAC 13.2 ± 8.5 2.6 ± 0.4
S1F cHb-A IMAC 17.0 ± 2.3 1.1 ± 0.1
S3R cHb Control 30.2 2.4
S3R cHb-D IMAC 19.8 ± 8.85 2.5 ± 0.3
S3R cHb-A IMAC 25.1 ± 5.73 1.6 ± 0.4