Fig. 4.

Effects of CLE10 overexpression on root growth and protoxylem vessel formation. The WT (Col-0) and XVE:CLE10-harboring seedlings were grown on agar plates (with 1.5% agar) for 7 d with (+) or without (−) 5 μM estradiol. (A) Inhibition of root growth by overexpression of CLE10. Scale bar is 5 mm. (B) Inhibition of protoxylem vessel formation by overexpression of CLE10. Black, gray and white bars are explained in Fig. 3 (N = 12–14). (C, D) Images of root vessels in the WT (C) and XVE:CLE10-harboring (D) seedlings, when treated with and without estradiol. Scale bars are 25 μm.