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Chalcopyrite bioleaching assays. Filled triangle, 5× >30 kDa subfraction of the total sulfur secretome of A. thiooxidans; empty circle, 5× <30 kDa subfraction of the total sulfur secretome of A. thiooxidans; empty diamond, control with the addition of A. thiooxidans heat-inactivated total secretome; empty square, control with no addition of secretome subfractions; filled diamond, control without inoculum. Left, percent Cu recovery; right, bacterial growth determinations
