TABLE 1 .
Tn-seq analysis of P. aeruginosaa
| Growth condition | Total no. of reads | No. (%) of mapped reads | No. of locations identified | No. (%) of authentic locationsb |
|---|---|---|---|---|
| Initial poolc | 19,778,278 | 15,739,802 (80) | 95,905 | 94,531 (98.6) |
| Trial 1 | ||||
| Without tobramycin | 14,152,566 | 12,601,670 (89) | 96,745 | 96,185 (99.4) |
| With tobramycin | 10,699,404 | 9,691,469 (91) | 76,474 | 76,109 (99.5) |
| Trial 2 | ||||
| Without tobramycin | 12,660,378 | 11,081,531 (88) | 99,580 | 98,258 (98.7) |
| With tobramycin | 12,041,290 | 10,799,523 (90) | 98,243 | 96,925 (98.7) |
a
A pool of approximately 100,000 independent transposon mutants was analyzed by the Tn-seq circle method after growth under different conditions.
b
Authentic locations were defined as those identified in multiple Tn-seq assays of samples derived from the transposon mutant pool.
c
The values for the initial pool represent two replicate assays of a single DNA preparation.