Skip to main content
. Author manuscript; available in PMC: 2011 Jan 20.
Published in final edited form as: Cell Transplant. 2010 Jun 29;19(11):1397–1412. doi: 10.3727/096368910X513955

Figure 6.

Figure 6

(A) Viability and live cell concentration (per ml of medium) from mESCs encapsulated in LC beads and cultured in spinner flasks. (B) Profile of LDH activity in the spinner flask cultures of encapsulated mESCs. (C) Expression of cardiac genes by LC bead-encapsulated mESCs differentiated in a bioreactor for 7 days. The expression of corresponding genes by mESCs differentiated in LC beads in static culture for the same period was used for comparison. Cells released from the beads at day 7 and plated for 2 days were stained for (D) cTnT (red) and (E) Nkx2.5 (green). Merged micrographs (D) and the last image to the right in (E) show DAPI costaining.