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. Author manuscript; available in PMC: 2011 Jan 20.
Published in final edited form as: Cell Transplant. 2010 Jun 29;19(11):1397–1412. doi: 10.3727/096368910X513955

Figure 7.

Figure 7

(A) Profiles of viability and live cell concentration for hESCs encapsulated in LC beads and cultured in spinner flasks. (B) Profile of LDH activity in the spinner flask culture of encapsulated hESCs. (C) Expression of cardiac genes by LC bead-encapsulated hESCs differentiated in a bioreactor for 2 weeks. (D) Fractions of Nkx2.5+ and GATA4+ cells resulting from the differentiation of hESCs encapsulated in LC beads and cultured in dishes (open bars) or spinner flasks (gray bars) for 2 weeks. Upon their release from the LC beads, hESC-derived aggregates form beating foci which respond to chronotropic agents such as (E) IBMX and (F) diltiazem. *p < 0.001 compared to baseline response.