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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Steroids. 2010 Nov 2;76(1-2):135–139. doi: 10.1016/j.steroids.2010.10.001

Fig. 1.

Fig. 1

Direct sequencing and restriction fragment length polymorphism assay (RFLP) results for the H6PD gene. The polymorphisms were detected by direct PCR sequencing and confirmed by RFLP: (A) An eight bp (acccaggc) deletion confirmed by Ban I digestion (The 68-bp fragment is not seen because it was too low on the gel. (B) Bbs I digestion confirms the c.452 genotype a/c, which encodes the D151A missense variant. (C) The Bsg I digestion confirms the c.1358 genotype g/a, which encodes the R453Q missense variant (the 179-bp fragment is not seen because it was too low on the gel). (D) The Ban II digestion confirms the c.1661 genotype c/t, which encodes the P554L missense variant (the 53 and 6-bp fragments are not seen because they were too low on the gel). Double and single underlines locate the homozygous and heterozygous acccaggc sequences, respectively; and arrows indicate the variants.