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. Author manuscript; available in PMC: 2011 Dec 16.
Published in final edited form as: Cell Host Microbe. 2010 Dec 16;8(6):534–543. doi: 10.1016/j.chom.2010.11.003

Figure 7.

Figure 7

APOBEC3G made in MECs is packaged into HIV. A) HS578T (left) and T47D (right) cells were transduced with PNL-Luc that contained (+ vif) or lacked (− vif) the vif gene and a vector bearing the VSV G protein. Twenty-four hrs post-transduction, progesterone was added to the media, as indicated (+) or left untreated (−) and 24 hr later, virus was harvested from the cell supernatants and used to infect NP2 cells, in the absence or presence of AZT, as indicated. In addition, p24 levels were measured by ELISA. Twenty-four after infection, luciferase assays were performed on lysates prepared from the NP2 cells. RLUs were normalized to the p24 levels in the virus preparations. Error bars represent SD from 3 replicate infections. *, NS; **, p ≤ 0.05; #, p ≤ 0.001. This experiment was performed 3 times with similar results. B) The virion preparations were analyzed by Western blots, using anti-p24 antisera. Virions prepared in PNL-Luc+vif-transduced 293T cells were included as positive controls.