Table 1.

Observed rate constants associated with glucose binding at different viscosities.

[Glucose] (mM)	% Glycerol (w/v) (relative viscosity)	kobs1	kobs2	kobs3	kobs4
96	0 (1.0)	57 ± 0.3	2.2 ± 0.02	0.75 ± 0.003	0.027 ± 0.0001
	5 (1.2)	44 ± 0.2	2.2 ± 0.01	0.77 ± 0.002	0.029 ± 0.0001
	10 (1.4)	43 ± 0.2	2.1 ± 0.01	0.70 ± 0.002	0.028 ± 0.0001
	20 (1.9)	35 ± 0.2	1.5 ± 0.01	0.54 ± 0.003	0.025 ± 0.0001
	40 (4.3)	16 ± 0.1	0.9 ± 0.01	0.20 ± 0.001	0.022 ± 0.0001
75	0 (1.0)	58 ± 0.4	2.4 ± 0.02	0.75 ± 0.002	0.026 ± 0.0001
	5 (1.2)	59 ± 0.3	2.6 ± 0.02	0.76 ± 0.002	0.028 ± 0.0001
	10 (1.4)	43 ± 0.2	2.2 ± 0.01	0.66 ± 0.001	0.026 ± 0.0001
	20 (1.9)	27 ± 0.2	1.5 ± 0.01	0.51 ± 0.001	0.024 ± 0.0001
	40 (4.3)	17 ± 0.2	0.9 ± 0.01	0.25 ± 0.001	0.020 ± 0.0001
48	0 (1.0)	59 ± 0.5	2.3 ± 0.02	0.66 ± 0.002	0.025 ± 0.0001
	5 (1.2)	38 ± 0.2	2.1 ± 0.02	0.62 ± 0.001	0.028 ± 0.0001
	10 (1.4)	35 ± 0.2	2.1 ± 0.01	0.56 ± 0.001	0.026 ± 0.0001
	20 (1.9)	27 ± 0.3	1.5 ± 0.02	0.44 ± 0.001	0.022 ± 0.0001
	40 (4.3)	17 ± 0.3	0.9 ± 0.02	0.22 ± 0.001	0.019 ± 0.0001
24	0 (1.0)	52 ± 0.7	2.1 ± 0.03	0.49 ± 0.001	0.025 ± 0.0001
	5 (1.2)	42 ± 0.3	2.5 ± 0.03	0.47 ± 0.001	0.027 ± 0.0001
	10 (1.4)	48 ± 0.3	2.4 ± 0.02	0.39 ± 0.001	0.025 ± 0.0001
	20 (1.9)	15 ± 0.2	1.2 ± 0.02	0.29 ± 0.001	0.022 ± 0.0001
	40 (4.3)	19 ± 0.2	0.9 ± 0.01	0.14 ± 0.001	0.018 ± 0.0001
12	0 (1.0)	34 ± 0.5	2.2 ± 0.04	0.38 ± 0.001	0.025 ± 0.0001
	5 (1.2)	45 ± 0.6	2.7 ± 0.03	0.35 ± 0.001	0.028 ± 0.0001
	10 (1.4)	36 ± 0.5	2.4 ± 0.02	0.27 ± 0.001	0.026 ± 0.0001
	20 (1.9)	7.0 ± 0.3	1.2 ± 0.05	0.18 ± 0.001	0.021 ± 0.0001
	40 (4.3)	9.0 ± 0.2	0.6 ± 0.01	0.09 ± 0.001	0.017 ± 0.0001
6	0 (1.0)	27 ± 0.5	1.8 ± 0.05	0.32 ± 0.001	0.028 ± 0.0002
	5 (1.2)	20 ± 0.3	1.6 ± 0.05	0.28 ± 0.001	0.031 ± 0.0002
	10 (1.4)	16 ± 0.3	1.6 ± 0.03	0.21 ± 0.001	0.028 ± 0.0001
	20 (1.9)	16 ± 0.4	1.3 ± 0.03	0.14 ± 0.001	0.024 ± 0.0001
	40 (4.3)	14 ± 0.7	0.8 ± 0.02	0.06 ± 0.001	0.015 ± 0.0001
3	0 (1.00)	20 ± 0.4	1.4 ± 0.08	0.30 ± 0.001	0.039 ± 0.001
	5 (1.2)	22 ± 0.6	1.5 ± 0.08	0.26 ± 0.001	0.046 ± 0.001
	10 (1.4)	3.0 ± 0.3	0.7 ± 0.34	0.18 ± 0.002	0.051 ± 0.001
	20 (1.9)	19 ± 0.8	1.0 ± 0.04	0.11 ± 0.001	0.035 ± 0.001
	40 (4.3)	18 ± 0.3	0.6 ± 0.014	0.05 ± 0.001	0.015 ± 0.0001