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. Author manuscript; available in PMC: 2011 Jan 20.
Published in final edited form as: Biochemistry. 2010 Oct 19;49(41):8902–8911. doi: 10.1021/bi1008672

Table 2.

Temperature dependence of the observed rate constants. Traces collected below 12 mM glucose at 10°C were not included due to their failure to be fitted to a four exponential function.

[Glucose] (mM) Temperature (°C) kobs1 kobs2 kobs3 kobs4
96 25 57 ± 0.3 2.2 ± 0.1 0.75 ± 0.003 0.027 ± 0.001
10 20 ± 3.4 2.2 ± 0.5 0.20 ± 0.003 0.015 ± 0.001

75 25 59 ± 0.4 2.4 ± 0.1 0.75 ± 0.002 0.026 ± 0.001
10 22 ± 3.6 2.8 ± 0.5 0.20 ± 0.002 0.017 ± 0.002

48 25 59 ± 0.5 2.3 ± 0.1 0.66 ± 0.002 0.025 ± 0.001
10 20 ± 2.9 2.7 ± 0.7 0.17 ± 0.002 0.021 ± 0.003

24 25 52 ± 0.6 2.1 ± 0.1 0.49 ± 0.001 0.025 ± 0.001
10 24 ± 2.6 2.7 ± 0.4 0.14 ± 0.003 0.048 ± 0.009

12 25 34 ± 0.5 2.2 ± 0.1 0.38 ± 0.001 0.025 ± 0.001
10 22 ± 0.9 3.7 ± 1.1 0.47 ± 0.17 0.102 ± 0.015