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. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Biochemistry. 2010 Sep 21;49(37):7969–7971. doi: 10.1021/bi101098f

FIGURE 3.

FIGURE 3

1H-15N HSQC NMR spectra of glucokinase with 15N tryptophan side chain labels in the absence (A) and presence (B) of glucose. The spectra were acquired as matrices of 2048(N2)×128(N1) or 2048(N2)×64(N1) real points and accumulation of 128 or 64 scans in the absence or presence of glucose, respectively. The indirect dimension of the HSQC spectrum of the glucose-bound sample was extended up to 128 points by linear prediction. All dimensions were multiplied by a cosine square apodization function and zero filled up to 2048(N2)×256(N1) data points. The spectrum in the absence of glucose was acquired using a smaller spectral window in the 15N dimension (813Hz compared to 2433 Hz in the presence of glucose).