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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Dev Dyn. 2011 Feb;240(2):432–440. doi: 10.1002/dvdy.22550

Figure 1.

Figure 1

Expression of Wnt5a in the developing tooth. (A, D) Wnt5a expression is detected by in situ hybridization mainly in the dental papilla of molar (A) and incisor (D) at E14.5. (B, E) Expression of Wnt5a is found in dental papilla of E16.5 molar (B) and incisor (E). Note in the molar (B) the expression is strongly localized in the areas immediately adjacent to the epithelial sites where the secondary enamel is forming. (C, F) At P0, Wnt5a expression is found restrictedly in the differentiating odontoblasts (arrows) of the molar (C) and incisor (F). Wnt5a expression is also detected in enamel epithelium, particularly in the cervical loop in the incisor at this stage (F). (G) Lack of Wnt5a expression in an E16.5 Wnt5a mutant molar, which serves as a negative control for in situ hybridization. All sections shown were made through coronal plane, except that in panels E and F were made through sagittal plane. Dot lines demarcate the boundary of dental epithelium. cl, cervical loop; De, dental epithelium; dp, dental papilla.