Figure 4.

Wnt5a is required for tooth development and patterning. (A, B) Whole mount view of the first molars from P0 wild type (A) and P0 Wnt5a mutant (B) reveals retarded tooth development and dis-regulated cusp patterning in the mutant. The mutant molar appears smaller and misses the lingual 1 (L1) and distal (d) cusps. All remaining cusps in the mutant are blunted. (C, D) After four weeks in subrenal culture, the mutant molar remains smaller and shows blunted cusps (arrows) (D), as compared to the wild type controls (C). (E–H) Wnt5a mutant incisor becomes largely shortened in proximal-distal length bluntly ended at P0 (F), and remains similarly morphologically after 4-week in subrenal culture (H), as compared to the wild type controls (E, G). B1-3 represents buccal side cusps; and L1-3 indicate lingual site cusps.