Table 1.
Densitometry scans of the phosphorylated (P1 and P2) Cx43‐bands relative to the unphosphorylated (P0) band from Western blots of proteins extracted from three different cell treatments in which one blot is presented in Figure 1(f)
| Treatment | Densitometry Ratios of P1 and P2 Relative to P0 (FOC) | |||
|---|---|---|---|---|
| 5 min | 10 min | 20 min | 30 min | |
| P0/P1 | ||||
| V | 1.00 ± 0.04 | 1.00 ± 0.18 | 1.00 ± 0.08 | 1.00 ± 0.08 |
| A | 0.90 ± 0.12 | 0.99 ± 0.28 | 0.94 ± 0.05 | 0.91 ± 0.23 |
| 1‐MeA | 1.01 ± 0.03 | 1.04 ± 0.12 | 1.05 ± 0.12 | 0.97 ± 0.15 |
| 2‐MeA | 0.93 ± 0.05 | 1.05 ± 0.25 | 0.62 ± 0.28 | 0.93 ± 0.02 |
| 9‐MeA | 1.00 ± 0.07 | 1.01 ± 0.12 | 0.91 ± 0.01 | 1.12 ± 0.05 |
| TPA | ND | † 0.34 ± 0.16 | ND | ND |
| ANOVA (F, P‐value) | (1.73, 0.218) | (4.718, 0.013) | (3.468, 0.05) | (1.233, 0.357) |
| P0/P2 | ||||
| V | 1.00 ± 0.04 | 1.00 ± 0.19 | 1.00 ± 0.14 | 1.00 ± 0.17 |
| A | 0.93 ± 0.13 | 0.94 ± 0.28 | 0.95 ± 0.13 | 0.86 ± 0.22 |
| 1‐MeA | 1.03 ± 0.06 | 1.00 ± 0.13 | 1.13 ± 0.07 | 1.16 ± 0.25 |
| 2‐MeA | 0.97 ± 0.16 | 1.01 ± 0.26 | 0.54 ± 0.25 | 1.00 ± 0.24 |
| 9‐MeA | 1.05 ± 0.09 | 0.94 ± 0.08 | 1.03 ± 0.15 | 1.26 ± 0.33 |
| TPA | ND | † 0.27 ± 0.07 | ND | ND |
| ANOVA (F, P‐value) | (0.613, 0.663) | (6.584, 0.004) | (2.642, 097) | (1.484, 0.278) |
†
Significant from the vehicle as determined by a Holm‐Sidak post hoc test. The ANOVA was conducted on the densitometry data, including the vehicle. The fraction of the control (FOC) is the densitometry value of each treatment divided by the densitometry value of the vehicle within each time group. 1‐MeA, 1‐methylanthracene; 2‐MeA, 2‐methylanthracene; 9‐MeA, 9‐methylanthracene; A, anthracene; Cx43, connexin43; ND, not determined; TPA, 12‐O‐tetradecanoylphorbol‐13‐acetate; V, vehicle.