Table 2.

Composition of ions in the MS² spectrum of the [M+H]⁺ ion of [Asp³]-MC-RY

Composition and sequence	Calculated m/z	Measured m/z	Error [ppm]
M (+ H+)	1031.5197	1031.5197	0.0
M (− CO) (+ H+)	1003.5248	1003.5244	−0.4
Glu-(Mdha or Dhb)-Ala-Arg-Asp (+ H+)	555.2522	555.2643	21.8
Glu-(Mdha or Dhb)-Ala-Arg (+ H+)	440.2252	440.2386	30.4
Glu-(Mdha or Dhb)-Ala-Arg (− COOH) (+ H+)	395.2276	395.2124	−38.5
Glu-(Mdha or Dhb) (+ H+)	213.0870	213.0879	4.2
(Mdha or Dhb)-Ala-Arg-Asp (+ H+)	426.2096	426.2177	19.0
(Mdha or Dhb)-Ala-Arg-Asp (− NH3) (+ H+)	409.1831	409.1936	25.7
(Mdha or Dhb)-Ala-Arg (+ H+)	311.1826	311.1879	17.0
(Mdha or Dhb)-Ala-Arg (− CO) (+ H+)	283.1877	283.1771	−37.4
(Mdha or Dhb)-Ala-Arg (− NH3) (+ H+)	294.1561	294.1605	15.0
(Mdha or Dhb)-Ala (+ H+)	155.0815	155.0824	5.8
(Mdha or Dhb)-Ala (− CO) (+ H+)	127.0866	127.0899	26.0
Ala-Arg-Asp-Tyr-Adda (+ H+)	819.4400	819.4226	−21.2
Ala-Arg-Asp (+ H+)	343.1724	343.1826	29.7
Ala-Arg (+ H+)	228.1455	228.1474	8.3
Arg-Asp-Tyr (+ H+)	435.1987	435.2142	35.6
Arg-Asp-Tyr (− COOH) (+ H+)	390.2010	390.1989	−5.4
Arg-Asp (+ H+)	272.1353	272.1411	21.3
Arg (+ H+)	157.1084	157.1098	8.9
Asp-Tyr (+ H+)	279.0975	279.1034	21.1
Asp-Tyr (− CO) (+ H+)	251.1026	251.1096	27.9
Tyr-Adda-Glu-(Mdha or Dhb)-Ala-Arg (+ H+)	916.4927	916.4935	0.9
Tyr-Adda-Glu-(Mdha or Dhb)-Ala-Arg (− CO) (+ H+)	888.4978	888.5056	8.8
Tyr-Adda-Glu-(Mdha or Dhb) (+ H+)	689.3545	689.3567	3.2
Tyr-Adda-Glu (+ H+)	606.3174	606.3105	−11.4
Tyr-Adda (+ H+)	477.2748	477.2565	−38.3
Adda-Glu-(Mdha or Dhb)-Ala-Arg-Asp (− NH3) (+ H+)	851.4298	851.4353	6.5
Adda-Glu-(Mdha or Dhb)-Ala-Arg-Asp (− NH3) (− 134 Adda) (+ H+)	717.3565	717.3604	5.4
Adda-Glu-(Mdha or Dhb) (− NH3) (+ H+)	509.2647	509.2621	−5.1
Adda-Glu-(Mdha or Dhb) (− NH3) (− 134 Adda) (+ H+)	375.1915	375.1996	21.6
Adda-Glu-(Mdha or Dhb) (− CO) (− NH3) (− 134 Adda) (+ H+)	347.1966	347.2035	19.9
Adda (− NH3) (− 134 Adda) (+ H+)	163.1118	163.1127	5.5
134Adda (+ H+)	135.0804	135.0824	14.8