Table 4.
Field samples of twelve Ugandan freshwater sites analyzed for cyanobacterial biovolume, Microcystis cell numbers, by PCR for the presence (+) or absence (−) of microcystin (MC) producers (HEP), mcyE, PC-IGS, mcyB of Microcystis and for MCs by HPLC. Only the plankton net samples from the site nos. 4, 5, 7, 10, and 11 were analyzed in April 2008.
| Sample ID | Site no. |
Filtered volume (mL) |
Cyanobacterial Biovolume (mm3 L−1) |
Microcystis (cells mL−1) |
HEP-PCR product |
Microcystis mcyE-F2/F8* PCR product |
PC-IGS PCR product |
McyB PCR product |
fg MC-LR equivalent cell−1 |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | M4 | J4 | A8 | ||
|
| |||||||||||||||||||||||||
| Depth Integrated samples | |||||||||||||||||||||||||
| Kiranzi swamp | 1 | 250 | 250 | − | − | − | − | − | − | − | − | − | − | − | − | ||||||||||
| Nyabikere Crater Lake | 2 | 400 | 700 | 4 | 0.4 | − | − | − | − | − | − | − | − | − | − | − | − | ||||||||
| Nkuruba Crater Lake | 3 | 500 | 500 | 3 | 1 | 5×103 | 5×103 | − | − | − | − | + | + | − | − | − | − | ||||||||
| Lake George | 4 | 25 | 50 | 88 | 60 | 4×105 | 7×105 | + | + | + | + | + | + | + | + | − | − | ||||||||
| Lake Edward (Katwe) | 5 | 150 | 150 | 53 | 57 | 2×105 | 2×105 | + | + | + | + | + | + | + | + | − | − | ||||||||
| Nkugute Crater Lake | 6 | 700 | 500 | ||||||||||||||||||||||
| Lake Mburo | 7 | 100 | 200 | 63 | 88 | 9×105 | 9×105 | + | + | + | + | + | + | + | + | − | − | ||||||||
| Lake Nabugabo | 8 | 250 | 250 | 5 | 5 | 5×104 | 2×104 | − | − | − | − | + | + | + | + | − | − | ||||||||
| Lake Victoria (Bunjako Bay) | 9 | 800 | 500 | 11 | 24 | 2×104 | 4×104 | − | − | − | − | + | + | + | + | − | − | ||||||||
| Lake Victoria (Murchison Bay) | 10 | 500 | 500 | 18 | 129 | 4×104 | 2×105 | + | + | + | + | + | + | + | + | − | − | ||||||||
| Lake Victoria (Napoleon Gulf) | 11 | 500 | 500 | 40 | 46 | 3×104 | 3×104 | + | + | + | + | + | + | + | + | − | − | ||||||||
| Jinja Pond | 12 | 200 | 19 | 3×104 | − | − | − | − | − | ||||||||||||||||
| Plankton Net samples | |||||||||||||||||||||||||
| Kiranzi swamp | 1 | 40 | 30 | Not applicable | − | − | − | − | − | − | − | − | − | − | − | − | |||||||||
| Nyabikere Crater Lake | 2 | 50 | 50 | ″ | − | − | − | − | − | − | − | − | − | − | − | − | |||||||||
| Nkuruba Crater Lake | 3 | 50 | 50 | ″ | − | − | − | − | − | − | − | − | − | − | |||||||||||
| Lake George | 4 | 25 | 50 | 60 | ″ | 3×106 | 3×106 | 6×106 | + | − | + | + | − | + | + | + | + | + | + | + | 8.1 | − | 9.6 | ||
| Lake Edward (Katwe) | 5 | 35 | 40 | 60 | ″ | 1×106 | 4×106 | 2×106 | + | + | + | + | + | + | + | + | + | + | + | + | 92.9 | 31.2 | 67.1 | ||
| Lake Mburo | 7 | 35 | 50 | 60 | ″ | 3×106 | 3×106 | 4×106 | + | − | + | + | − | + | + | + | + | + | + | + | 5.2 | 15.8 | 52.4 | ||
| Lake Nabugabo | 8 | 40 | 40 | ″ | 3×105 | 5×105 | − | − | − | − | + | + | + | + | − | − | |||||||||
| Lake Victoria (Bunjako Bay) | 9 | 50 | 50 | ″ | 2×105 | 2×105 | − | − | − | − | + | + | + | + | − | − | |||||||||
| Lake Victoria (Murchison Bay) | 10 | 50 | 25 | 60 | ″ | 4×105 | 4×106 | 1×107 | + | + | + | + | + | + | + | + | + | + | + | + | − | − | 1.3 | ||
| Lake Victoria (Napoleon Gulf) | 11 | 50 | 50 | 60 | ″ | 1×106 | 7×105 | 6×105 | + | + | + | + | + | + | + | + | + | + | + | + | − | − | 49.1 | ||
| Jinja Pond | 12 | 50 | ″ | ||||||||||||||||||||||
M4 = May 2004; J4 = June 2004; A8 = April 2008;
*
using mcyE-F2/12R for Anabaena and plaR3 for Planktothrix no PCR products were obtained