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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Chem Biol Drug Des. 2010 May 11;76(2):164–173. doi: 10.1111/j.1747-0285.2010.00988.x

Figure 3. B3CD effect on cell proliferation and cell cycle progression in NB cells.

Figure 3

(A) Cell Cycle Analysis by FACS. Summary of previously published data (14).

(B) Expression of cyclin D1 and Cyclin-Dependent Kinase inhibitors in B3CD treated NB cells. Expression of cyclin D1, p21 and p27 cell cycle regulators in B3CD and vehicle treated SMS-KCNR or SH-SY5Y cells were analyzed by western blotting of lysates with the appropriate primary and secondary antibodies in combination with a chemiluminescence detection system as described in (Materials and Methods). As an internal standard for equal loading (50 μg total cell protein/lane) blots were probed with an anti-GAPDH antibody.

(C) Semi-quantitative RT-PCR of cyclin D1 and Cyclin-Dependent Kinase inhibitors in B3CD treated NB cells. Total RNA was isolated from SMSK-CNR or SH-SY5Y cells treated with 0, 1 and 3 μM of B3CD for 48 h. RT PCR was carried out as described (Material and Methods).