Fig. 2.

a Expression of KLF4 protein in KLF4-overexpressed K562 cells and PBMCs. K562 cells and PBMCs were transfected with pcDNA3.1-KLF4, and the expression level of KLF4 was identified by Western blot analysis. Neo vector control group, KLF4 KLF4 overexpression group. b Effect of KLF4 overexpression on cell proliferation determined by CCK-8. PNeo vector control group of PBMCs, PKLF4 KLF4 overexpression group of PBMCs, KNeo vector control group of K562 cells, KKLF4 KLF4 overexpression group of K562 cells. The relative values of all results were determined and expressed as the mean±SEM of three experiments performed in duplicate. *P < 0.05. c Effect of KLF4 overexpression on the cell apoptosis rate determined by flow cytometry. PNeo vector control group of PBMCs, PKLF4 KLF4 overexpression group of PBMCs, KNeo vector control group of K562 cells, KKLF4 KLF4 overexpression group of K562 cells. The relative values of all results were determined and expressed as the mean±SEM of three experiments performed in duplicate. *P < 0.05. d Effect of KLF4 overexpression on caspase-3 activity assayed using caspase fluorescence assay kits. PNeo vector control group of PBMCs, PKLF4 KLF4 overexpression group of PBMCs, KNeo vector control group of K562 cells, KKLF4 KLF4 overexpression group of K562 cells. The relative values of all results were determined and expressed as the mean±SEM of three experiments performed in duplicate. *P < 0.05. e Effect of KLF4 overexpression on the levels of cleaved caspase-3 and PARP measured by Western blot analysis. PNeo vector control group of PBMCs, PKLF4 KLF4 overexpression group of PBMCs, KNeo vector control group of K562 cells, KKLF4 KLF4 overexpression group of K562 cells. Cells were treated with H₂O₂ (0.5 mmol/l) for 48 h. The relative values of all results were determined and expressed as the mean±SEM of three experiments performed in duplicate. *P < 0.05