Fig. 3.

CCT-eta mRNA and protein levels remain elevated in healing adult wounds but CCT-beta levels remain constant. a RNA extracted from 12 h post-injury until 28 day post-injury from adult wounds was subjected to qRT-PCR analysis. CCT-eta mRNA levels gradually increased from 12 h and remain elevated until Day 28. In contrast, there was no significant difference in CCT-beta mRNA levels due to wounding. Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student’s t test **p < 0.01; ***p < 0.001. b Equal amounts of protein loaded from post-injury time point 12 h and Day 28 showed substantial increase in CCT-eta protein levels, but CCT-beta protein remained constant. c Densitometry analysis of protein bands shows an increase in CCT-eta protein accumulation with no corresponding change in CCT-beta protein expression at 12 h and Day 28. GAPDH was used as the internal loading control. Blots shown here are representative of at least three different experiments. AC-adult control; AW-adult wound