Skip to main content
. 2010 Apr 15;15(6):819–826. doi: 10.1007/s12192-010-0190-1

Table 2.

Primer and probe sequences used for quantitative real time RT-PCR

Gene Sequences
Rabbit CCT-eta Forward 5′-GTGGTGCTGAGCAGTTTATGGA-3′
Rabbit CCT-eta Reverse 5′-TTCTTAATGGCCCTCCTGACA-3′
Rabbit CCT-eta Probe 5′-6 FAM- AGACGGAGCGGTCCCTGCATGA-TAMRA-3′
Rabbit CCT- beta Forward 5′-CATCAAAGCAGCACCAAGGA-3′
Rabbit CCT- beta Reverse 5′- TGGTCCAGAGATCAAGATGCAT-3′
Rabbit CCT- beta Probe 5′- 6 FAM -TGATCACCATCCTTGTTAAGCATACCCATATAMRA-3′
Rabbit GAPDH Forward 5′- CGCCTGGAGAAAGCTGCTAA-3′
Rabbit GAPDH Reverse 5′- CTCGGTGTAGCCCAGGAT-3′
Rabbit GAPDH Probe 5′-6 FAM-AAGCAGGCATCCGAGGGCCC-TAMRA-3′
Rabbit α-SMA Forward 5′-GACAGCTACGTGGGTGATGAAGC-3′
Rabbit α-SMA Reverse 5′-TCGTCCCAGTTGGTGATGATG-3′
Rabbit α-SMA Probe 5′-6 FAM-ACCTTGAAGTACCCGATCGAACATGGC-TAMRA-3′

The primers and probes were designed using PRIMER EXPRESS® software v2.0 provided by Applied Biosystems.