Fig. 1.

Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10⁵/ml in phenol red-free DMEM supplemented with 5% charcoal absorbed FBS, and then indicated celastrol doses were added for 6 or 24 h. The cell's viability was determined by MTT method. Upper panel exposure to celastrol for 6 h. Lower panel exposure to celastrol for 24 h. Each value represents mean ± SD from three independent tests