Table 1.

Statistics of TFs in HepG2 and THP-1 caused by celastrol

Cell line	HepG2			THP-1
Cel (nM)	100	600	3,000	100	600	1,000
ERα	1.05 ± 0.07	0.97 ± 0.05	0.94 ± 0.08	1.11 ± 0.06*	1.03 ± 0.05	0.3 ± 0.05**
ERβ	0.95 ± 0.06	0.98 ± 0.03	0.95 ± 0.05	ND	ND	ND
AR	1.03 ± 0.08	1.02 ± 0.05	1.05 ± 0.06	1.05 ± 0.07	0.99 ± 0.05	0.84 ± 0.03**
GR	0.96 ± 0.05	0.94 ± 0.07	1.01±0.04	1.08 ± 0.06	2.04 ± 0.06**	2.17 ± 0.03**
PPARα	ND	ND	ND	ND	ND	ND
PPARβ	0.66 ± 0.07**	1.09 ± 0.08	1.04 ± 0.06	ND	ND	ND
CAR	ND	ND	ND	1.44 ± 0.03**	1.8 ± 0.04**	2.11 ± 0.02**
PXR	1.85 ± 0.05**	0.97 ± 0.05	0.31 ± 0.03**	ND	ND	ND
HIF-1α	0.64 ± 0.03**	0.76 ± 0.02**	0.6 ± 0.02**	0.81 ± 0.02**	1.02 ± 0.04	0.84 ± 0.03**
HIF-2α	1.04 ± 0.08	0.65 ± 0.05**	0.51 ± 0.02**	0.93 ± 0.08	0.15 ± 0.06**	0.01 ± 0.01**
HIF-3α	1.01 ± 0.06	0.97 ± 0.05	0.94 ± 0.08	1.02 ± 0.05	0.93 ± 0.08	0.82 ± 0.06**
AhR	1.31 ± 0.02**	0.97 ± 0.05	0.68 ± 0.01**	0.77 ± 0.04**	0.27 ± 0.03**	0.02 ± 0.01**
Sim2	0.99 ± 0.05	1.00 ± 0.03	0.94 ± 0.07	0.65 ± 0.05**	0.63 ± 0.06**	0.29 ± 0.03**
IRF3	ND	ND	ND	ND	ND	ND
Stat3	1.01 ± 0.04	1.06 ± 0.08	1.02 ± 0.05	1.03 ± 0.09	1.01 ± 0.05	1.07 ± 0.08
P53	1.32 ± 0.06**	1.13 ± 0.04**	1.59 ± 0.03**	0.96 ± 0.07	0.98 ± 0.05	0.37 ± 0.03**
HSF-1	1.25 ± 0.04**	0.77 ± 0.02**	0.81 ± 0.03**	1.18 ± 0.05**	0.7 ± 0.03**	0.02 ± 0.01**
TonEBP	1.21 ± 0.03**	1.13 ± 0.05**	0.51 ± 0.05**	0.89 ± 0.02**	0.75 ± 0.03**	0.18 ± 0.02**

All values are represented as mean ± SD; experiments were independently repeated at least three times and tested relative to DMSO. ND not detectable

*P < 0.05; **P < 0.01