Table 1.

Putative mRNA targets for annotated thermally up-regulated microRNAs (TURMs)

TURM	Putative mRNA targets				Microcosma		PITA: thermodynamic modelb			Tarbasec
	Symbol	Name	Sequence	Function	Score	ΔGduplex	ΔGduplex	ΔGopen	ΔΔG	Validation
miR-125b	OSBPL9	Oxysterol-binding protein-related protein 9	NM_024586	Intracellular lipid receptor	17.1	−21.3	−18.9	−9.6	−9.3	No
	TTC7A	Tetratricopeptide repeat protein 7A	NM_020458	Chaperones (White et al.)	18.9	−29.1	−27.4	−13.6	−13.8	No
	ZFYVE1	Zinc finger FYVE domain containing protein	NM_021260	Recruitment of proteins and membrane trafficking	16.6	−28.0	−25.5	−9.6	−15.9	No
	DNAJB2	DnaJ homolog subfamily B member 2	NM_006736	Minimal stress protein (Kultz et al.)	16.7	−21.5	−26.0	−15.2	−10.8	No
	AGO2	Eukaryotic translation initiation factor 2C2	NM_012154	RNAinterference, role in CSR (Liu et al.)	15.9	−15.7	−13.7	−5.7	−8.0	No
miR-452	IGF1R	Insulin-like growth factor 1 receptor	NM_000875	Resistance to oxidative stress (Holzenberger et al.)	16.8	−19.1	−19.2	−9.1	−10.1	No
	POLR2B	Polymerase (RNA) II polypeptide B, 140 kDa	NM_000938	UV sensitive gene (Jung et al.)	15.8	−14.8	NR	NR	NR	No
	NOX3	NADPH oxidase 3	NM_015718	Generation reactive oxygen species (Li et al.)	19.9	−11.9	−15.8	−5.5	−10.3	No
	MPHOSPH9	M-phase phosphoprotein 9	NM_022782	Cellular physiological process	16.7	−13.8	−13.6	−6.9	−6.7	No
	TTC7A	Tetratricopeptide repeat protein 7A	NM_020458	Chaperones (White et al.)	18.2	−15.8	−15.7	−10.4	−5.3	No
miR-382	KYNU	Kynureninase (l-kynurenine hydrolase)	NM_001032998	Biosynthesis of NAD+	18.2	−14.2	NR	NR	NR	No
	HMGN3	High mobility group nucleosomal binding domain 3	NM_004242	Ethanol-induced apoptosis (Castenada et al.)	17.5	−15.3	−13.2	−7.5	−5.7	No
	DTYMK	Deoxythymidylate kinase	NM_012145	Metabolism and cytoxicity (Hu et al.)	18.5	−21.5	NR	NR	NR	No
	BCL7A	B-cell CLL/lymphoma 7A	NM_001024808	Hypoxia (van der Meer et al.)	NR	NR	−18.7	−6.8	−11.9	No
	PREPL	Prolyl endopeptidase-like	NM_006036	Metabolism, proteolysis	NR	NR	−16.6	−6.1	−10.5	No
miR-378	KLK4	Kallikrein-related peptidase 4	NM_004917	Metabolism (Deraison et al.)	17.6	−22.5	−21.0	−6.2	−14.8	No
	SUFU	Suppressor of fused homolog (Drosophila)	NM_016169	Enhances cell survival	NR	NR	−14.4	−8.1	−6.3	Lee et al.
	AHSA1	Activator of 90 kDa HSP ATPase	NM_012111	Response to stress (Amundson et al.)	17.7	−24.2	−21.4	−14.6	−6.8	No
	TTC7A	Tetratricopeptide repeat protein 7A	NM_020458	Chaperones (White et al.)	17.2	−25.5	−21.1	−16.7	−4.4	No
	IGF1R	Insulin-like growth factor 1 receptor	NM_000875	Resistance to oxidative stress (Holzenberger et al.)	15.8	−19.4	−20.1	−6.0	−14.2	No
miR-101	CDYL	Chromodomain protein, Y-like	NM_004824	DNA packaging protein	16.1	−16.5	−14.0	−9.8	−4.2	No
	USP47	Ubiquitin specific peptidase 47	NM_017944	Peptidase activity	17.6	−15.1	−15.9	−8.4	−7.5	No
	UBE2A	Ubiquitin-conjugating enzyme E2A	NM_003336	Proteolysis	15.4	−15.4	−13.3	−4.3	−9.0	No
	HSP90AB1	Heat shock protein 90-beta	NM_007355	Minimal stress protein	16.8	−11.3	−11.6	−11.2	−0.4	No
	MYCN	N-myc proto-oncogene protein	NM_005378	Positive regulator cellular proliferation	16.1	−13.5	−12.8	−7.7	−5.1	Lewis et al.
miR-424	STXBP3	Syntaxin binding protein 3	NM_007269	Cellular physiological process	17.0	−17.4	−14.3	−7.5	−6.8	No
	LRP6	Lipoprotein receptor-related protein 6	NM_002336	Morphogenesis	18.8	−18.7	−20.5	−11.7	−8.8	No
	HSPA4L	Heat shock 70 kDa protein 4-like	NM_014278	Chaperone protein (Kojima et al.)	16.5	−12.9	−13.8	−9.6	−4.2	No
	DNAJB4	DnaJ homolog subfamily B member 4	NM_007034	Minimal stress protein	15.8	−13.4	−16.5	−8.6	−7.9	No
	VWF	von Willebrand factor precursor	NM_000552	Stress response	17.5	−24.5	−19.8	−16.9	−2.9	No

^amiRanda MicroCosm: http://microrna.sanger.ac.uk/index.shtml

^bPITA: http://genie.weizmann.ac.il/pubs/mir07/mir07_data.html

^cTarBase v.5c: http://diana.cslab.ece.ntua.gr/tarbase/