Table 2.

Putative mRNA targets for annotated thermally down-regulated microRNAs (TDRMs)

TDRM	Putative mRNA targets				Microcosma		PITA: thermodynamic modelb			Tarbasec
	Symbol	Gene name	Sequence	Function	Score	ΔGduplex	ΔGduplex	ΔGopen	ΔΔG	Validation
miR-138	TMTC4	Transmembrane and tetratricopeptide repeat containing 4	NM_032813	Not well characterized	16.1	−20.6	−22.4	−8.9	−13.5	No
	CCT5	Chaperonin containing TCP1, subunit 5 (epsilon)	NM_012073	Chaperonin (TRiC complex)/protein renaturation	16.4	−23.8	−25.4	−6.0	−19.4	No
	TERT	Telomerase reverse transcriptase	NM_198253	Morphogenesis	16.4	−26.8	−28.3	−9.3	−19.0	Mitomo et al.
	AGO2	Eukaryotic translation initiation factor 2C2	NM_012154	RNAinterference, role in CSR (Liu et al.)	15.8	−29.9	−28.9	−10.8	−18.1	No
	HSPA4L	Heat shock 70 kDa protein 4-like	NM_014278	Hypertonic and heat stress (Kojima et al.)	16.9	−18.4	NR	NR	NR	No
miR-376a	MAN1C1	Mannosidase, alpha, class 1C, member 1	NM_020379	Metabolic processes	16.8	−18.0	−20.3	−10.0	−10.3	No
	SLC35F4	Solute carrier family 35, member F4	NM_001080455	Membrane transport	15.4	−17.0	−15.1	−8.3	−6.8	No
	ZCCHC7	Zinc finger, CCHC domain containing 7	NM_032226	Ion binding	17.1	−13.8	−11.2	−8.4	−2.8	No
	AGO2	Eukaryotic translation initiation factor 2C2	NM_012154	RNAinterference, role in CSR (Liu et al.)	15.9	−12.8	−13.4	−5.4	−8.0	No
	HSPA6	HSP70 protein 6	NM_002155	Minimal stress protein (Kultz et al.)	16.7	−12.7	−13.9	−12.7	−1.2	No
miR-let-7c	TRIM71	Tripartite motif-containing 71	NM_001039111	Development	16.4	−19.6	−16.5	−5.2	−11.3	Lin et al.
	HSPB2	HSP beta-2	NM_001541	Small HSP, minimal stress protein (Kultz et al.)	17.2	−15.5	−18.3	−11.7	−6.6	No
	GALE	UDP-galactose-4-epimerase	NM_000403	Metabolism	17.6	−23.2	−21.4	−4.3	−17.1	No
	IGF1R	Insulin-like growth factor 1 receptor	NM_000875	Resistance to oxidative stress (Holzenberger et al.)	NR	NR	−18.3	−4.2	−14.1	No
	RNF20	Ring finger protein 20	NM_019592	Metabolism	17.0	−24.8	−23.5	−9.0	−14.5	No
miR-196a	HOXB8	Homeobox B8	NM_024016	Differentiation	20.9	−39.5	−36.1	−4.3	−31.8	Kawasaki et al.
	MYC	V-myc myelocytomatosis viral oncogene homolog	NM_002467	Regulation physiological process	17.2	−17.5	−17.1	−11.6	−5.5	No
	HOXC8	Homeobox C8	NM_022658	Cell cycle progression (Kamel et al.)	18.0	−34.8	−31.7	−9.4	−22.3	Yekta et al.
	HSPH1	HSP 105 kDa (HSP 110 kDa)	NM_006644	Minimal stress protein (Kultz et al.)	16.3	−19.3	NR	NR	NR	No
	MAPKAPK5	MAP kinase-activated protein kinase	NM_139078	Minimal stress protein	16.1	−13.5	NR	NR	NR	No
miR-196b	HOXB8	Homeobox B8	NM_024016	Differentiation	20.1	−34.6	−31.8	−4.3	−27.5	Yekta et al.
	MYC	V-myc myelocytomatosis viral oncogene homolog	NM_002467	Regulation physiological process	16.8	−17.2	−15.0	−11.6	−3.4	No
	RNF20	Ring finger protein 20	NM_019592	Metabolism	15.9	−16.0	−17.0	−9.0	−8.0	No
	AKR1B1	Aldo-keto reductase family 1, member B1	NM_001628	Response to stress	15.7	−15.9	NR	NR	NR	No
	HOXC8	Homeobox C8	NM_022658	Cell cycle progression (Kamel et al.)	17.2	−32.0	−30.0	−9.4	−20.6	Yekta et al.

^amiRanda MicroCosm: http://microrna.sanger.ac.uk/index.shtml

^bPITA: http://genie.weizmann.ac.il/pubs/mir07/mir07_data.html

^cTarBase v.5c: http://diana.cslab.ece.ntua.gr/tarbase/