Table 1.
Oligonucleotide primers used for cloning and expression analysis of VpsHSPs
| Primer | Sequence (5′–3′) | Sequence information |
|---|---|---|
| P1(reverse) | CCATCAGTGAAATGTAAAAGCAA | 5′RACE primer for VpsHSP-1 |
| P2(reverse) | ATTGATGTCAGACGAAGAGGATC | 5′RACE primer for VpsHSP-1 (nested primer) |
| P3(reverse) | TTCTACCTTCCCTTGAGCTATTC | 5′RACE primer for VpsHSP-2 |
| P4(reverse) | TTCTTCTTTACCCTCTTTGGACT | 5′RACE primer for VpsHSP-2 (nested primer) |
| P5(forward) | TTAGAAAGAATCAGAAAGGACAT | Full-length verified primer for VpsHSP-1 |
| P6(reverse) | TTTTTTTTTTTTTTTTTTTTTT | Full-length verified primer for VpsHSP-1 and VpsHSP-2 |
| P7(forward) | TGATCGTCAGCGAGATATGTTCT | Full-length verified primer for VpsHSP-2 |
| P8(forward) | TTTATTTTCGGACTGGGTCAAGG | Real-time primer for VpsHSP-1 |
| P9(reverse) | GGTCTGTCACAAATGGCTTCTC | Real-time primer for VpsHSP-1 |
| P10(forward) | AGGGTACGTGATGAGATGCACAG | Real-time primer for VpsHSP-2 |
| P11(reverse) | GTAACACTCCATCCTCCGACAAG | Real-time primer for VpsHSP-2 |
| P12(forward) | CTCCCTTGAGAAGAGCTACGA | Real-time actin primer |
| P13(reverse) | GATACCAGCAGATTCCATACCC | Real-time actin primer |