Protein expression levels of HOP, HSC70, HSP90, and actin in tumor and non-tumor tissues from colon cancer patients. Soluble proteins were extracted from the tumor and non-tumor tissues of the same patients. These proteins (2 μg/lane for HSP90 and HSC70 or 1 μg/lane for HOP and β-actin) were separated by SDS-PAGE and analyzed by western blotting using the rabbit antibody to HSP90, rat antibody to HSC70, rabbit antibody to HOP, or mouse antibody to β-actin. Tissue samples from 19 colon cancer patients were tested. N non-tumor tissue, T tumor tissue