Fig. 2.

The NF-κB complex activated following exercise is composed of p50 and p65. Rats were exercised at 30 m/min for 20 min, hearts removed and processed for EMSA supershifts as described in “Materials and methods”. Shown here is a portion of an autoradiogram. Lane 1, control (no running). Lane 2, 30 m/min, no recovery. Lane 3, 30 m/min, no recovery plus p50 antibody. Lane 4, 30 m/min, no recovery plus p65 antibody. Lane 5, 30 m/min, no recovery plus RelB antibody. Lane 6, 30 m/min, no recovery plus c-Rel antibody. Lane 7, 30 m/min, no recovery plus Bcl-3 antibody. Lane 8, purified p50 protein. Lane 9, purified p50 protein plus p50 antibody. p65 and the fast and slow-migrating p50 NF-κB subunits are identified