Fig. 3.

High-intensity exercise activates AP-1. Protein extracts from hearts from animals exercised at 30 m/min for 20 min were incubated with a ³²P-labeled AP-1 binding sequence and analyzed by EMSA as described in “Materials and methods”. Shown here is a portion of a representative autoradiogram. Lane 1, control, (no running). Lane 2, 20 m/min, no recovery. Lane 3, 20 m/min, 2 h recovery. Lane 4, 20 m/min, 24 h recovery. Lane 5, 30 m/min, no recovery. Lane 6, 30 m/min, 2 h recovery. Lane 7, 30 m/min, 24 h recovery. AP-1 is identified