Fig. 1.

Relative mRNA expression profiles for Pv-hsf1 and Pv-hsp90 in heat-shocked (a, d) and anhydrobiotic (b, e) chironomid larvae. Values for the mRNA level of each gene were corrected with PvEf1-alpha expression level. The level of expression was calculated for each gene relative to the expression in control hydrated larvae (value = 1). Error bars represent mean value ± 95% CI for three replicates. cont. control hydrated larvae. c, e Neighbor-joining tree of the Pv-HSF1 and Pv-HSP90 amino acid sequences