Figure 1.

Transcript abundance and histone modifications after priming and potentiated activation of three WRKY transcription factor genes. Plants were treated with 100 μM BTH or wettable powder (control). After 72 h, half of the plants were stressed by infiltrating water into their leaves. After 3 h, leaves were collected and RNA and chromatin were isolated. (A–C) Transcript abundance as determined by RT–qPCR. Data are standardized for abundance of the Actin2 transcript. (D–F) H3K4 methylation (me) and histone acetylation (ac) on the gene promoters. Data are standardized for histone modification levels in the absence of inducer and stress treatment. Each data point is based on four independent replicates. Error bars indicate s.e.m. values. BTH, acibenzolar S-methyl; RT–qPCR, reverse transcriptase–quantitative PCR.