Table 2.
Overview of DNA concentrations using Nanodrop and Gel based estimation
| Sample ID* |
Nanodrop conc (ng/μl) |
260/280 ratio |
260/230 ratio |
Gel-based conc (ng/μl) |
Correction factor** |
Array QC*** |
|---|---|---|---|---|---|---|
| 1 | 131.0 | 1.84 | 2.47 | 134.5 | 0.97 | OK |
| 2 | 153.0 | 1.84 | 2.42 | 130.7 | 1.17 | OK |
| 3 | 31.9 | 1.88 | 2.67 | 24.9 | 1.28 | OK |
| 4 | 10.9 | 1.66 | 0.41 | 4.5 | 2.41 | NP |
| 5 | 13.6 | 2.08 | 1.57 | 3.9 | 3.51 | NP |
| 6 | 44.7 | 1.8 | 1.38 | 20.5 | 2.18 | NP |
| 7 | 9.8 | 2.06 | 1.92 | 9.0 | 1.08 | NP |
| 8 | 44.0 | 1.1 | 0.3 | 3.1 | 14.36 | NP |
| 9 | 57.5 | 1.57 | 0.82 | 3.8 | 14.94 | Poor |
| 10 | 540.0 | 1.78 | 1.9 | 346.9 | 1.56 | OK |
| 11 | 164.0 | 1.63 | 1.24 | 19.5 | 8.42 | Poor |
| 12 | 102.0 | 1.76 | 1.98 | 19.3 | 5.27 | OK |
| 13 | 485.0 | 1.84 | 2.34 | 270.5 | 1.79 | OK |
| 14 | 291.0 | 1.8 | 2.3 | 127.8 | 2.28 | OK |
| 15 | 430.0 | 1.69 | 2.17 | 76.3 | 5.64 | Poor |
| 16 | 208.0 | 1.81 | 2.21 | 135.9 | 1.53 | OK |
| 17 | 269.0 | 1.84 | 2.35 | 192.3 | 1.40 | OK |
| 18 | 63.0 | 1.73 | 2.11 | 9.7 | 6.48 | Poor |
| 19 | 215.0 | 1.75 | 2.12 | 146.6 | 1.47 | OK |
| 20 | 285.0 | 1.7 | 2.37 | 170.4 | 1.67 | OK |
| 21 | 1376.0 | 1.78 | 1.28 | 565.6 | 2.43 | OK |
| 22 | 88.6 | 1.67 | 0.91 | 68.1 | 1.30 | OK |
| 23 | 259.8 | 1.8 | 1.75 | 286.8 | 0.91 | OK |
| 24 | 496.4 | 1.7 | 1.8 | 523.9 | 0.95 | OK |
| 25 | 30.6 | 1.7 | 1.6 | 25.2 | 1.22 | NP |
| 26 | 172.0 | 1.8 | 2.11 | NP | NP | OK |
| 27 | 306.0 | 1.75 | 1.98 | NP | NP | OK |
| 28 | 203.0 | 1.89 | 2.31 | NP | NP | OK |
| 29 | 132.9 | 1.78 | 2.05 | NP | NP | OK |
| 30 | 300.0 | 1.8 | 1.96 | NP | NP | OK |
| 31 | 71.0 | 1.67 | 0.89 | NP | NP | OK |
| 32 | 47.5 | 1.71 | 0.68 | NP | NP | Poor |
| C1 | 500 | 1.8 | 1.95 | 462.9 | 1.08 | NP |
| C2 | 250 | 1.8 | 1.95 | 238 | 1.05 | NP |
| C3 | 50 | 1.8 | 1.95 | 45 | 1.11 | NP |
* Sample ID corresponds to the sample label in Figure 1
** Nanodrop concentration/Gel-based concentration
*** NP: Not performed