Figure 2. crm is limiting for distinct processes at low and high temperature.

Analysis with the null mutant crm³² of crm limiting roles on development at different temperatures (A–C). Morphological defects observed in crm mutants are temperature sensitive: ectopic sex combs (up, red arrows) are enhanced at high temperature (A) whereas abdominal dorsal fusion (middle, arrows) are observed only at low temperature (B). Wing margin defects (bottom, arrows) are enhanced at low temperature (C). The wing is from a hemyzygote escapers that can occasionally be obtained at 25°C. D: quantification of ectopic sex comb teeth (+/−1 standard deviation) in crm³² mutants at 18, 25 and 29°C in the distal sex comb on the second tarsal segment of the first leg, posterior sex comb on the second leg (L2) and the third leg (L3).