Table 2.
Effect of dietary milk phospholipids in the form of phospholipid-rich dairy milk extract (PLRDME) on liver and fecal lipid parameters in mice given an intragastric gavage of radioactively labelled cholesterol and sitostanol after 3 or 5 weeks on high-fat diet
| 3 weeks | 5 weeks | Combined | |||||||
|---|---|---|---|---|---|---|---|---|---|
| HF | HFPL | difference | HF | HFPL | difference | HF | HFPL | difference | |
| (n = 10) | (n = 8) | (n = 9) | (n = 10) | (n = 19) | (n = 18) | ||||
| Liver | |||||||||
| Total lipid (mg/organ) | 118 ± 6 | 89 ± 10 * | -25% | 212 ± 19 | 126 ± 7 *** | -41% | 162 ± 14 | 110 ± 7 ** | -32% |
| Cholesterol (μmol/organ) | 33 ± 1 | 26 ± 3 * | -21% | 64 ± 8 | 39 ± 4 ** | -39% | 48 ± 5 | 33 ± 3 * | -31% |
| Triglyceride (μmol/organ) | 65 ± 5 | 42 ± 8 * | -35% | 168 ± 14 | 90 ± 10 *** | -47% | 114 ± 14 | 70 ± 9 * | -38% |
| [14C]cholesterol (dpm/organ) | 6819 ± 836 | 3737 ± 948 * | -45% | 13560 ± 1737 | 9021 ± 1043 * | -34% | 10012 ± 1204 | 6673 ± 943 * | -33% |
| [3H]sitostanol (dpm/organ) | 317 ± 31 | 244 ± 52 | -23% | 547 ± 41 | 370 ± 38 ** | -32% | 426 ± 37 | 318 ± 34 * | -25% |
| Feces | |||||||||
| Total lipid (mg/mouse/day) | 6.4 ± 0.6 | 10.6 ± 0.7 *** | +66% | 9.2 ± 1.0 | 10.2 ± 1.1 | +11% | 7.8 ± 0.7 | 10.4 ± 0.7 * | +33% |
| Cholesterol (μmol/mouse/day) | 7.3 ± 0.4 | 9.1 ± 0.5 * | +25% | 6.7 ± 0.3 | 7.4 ± 0.4 | +11% | 7.0 ± 0.3 | 8.1 ± 0.4 * | +16% |
| Triglyceride (μmol/mouse/day) | 0.9 ± 0.1 | 1.0 ± 0.2 | +11% | 2.6 ± 0.5 | 2.0 ± 0.4 | -24% | 1.8 ± 0.3 | 1.6 ± 0.3 | -11% |
| [14C]cholesterol (dpm/mouse/day) | 9195 ± 1187 | 13454 ± 996 * | +46% | 8889 ± 795 | 10245 ± 864 | +15% | 9050 ± 711 | 11765 ± 740 * | +30% |
| [3H]sitostanol (dpm/mouse/day) | 12376 ± 1275 | 17110 ± 712 ** | +38% | 16076 ± 570 | 18134 ± 777 | +13% | 14129 ± 828 | 17679 ± 535 ** | +25% |
Results represent means ± SEM. Mice given the high-fat diet alone were designated HF. Mice given the high-fat diet supplemented with 2.5% by wt PLRDME were designated HFPL. Data are provided for animals fed for 3 or 5 weeks. Combined data are shown on the right. Percentage difference between groups is given in italics. Significant difference between HF and HFPL by Student's t-test: * P < 0.05, ** P < 0.01, *** P < 0.001.