Figure 6. Analysis of the rate of NC-eGFP release from single viruses fusing with endosomes.

(A) The NC-eGFP release from representative EnvA-pseudotyped virus particles upon treatment with 0.1 mg/ml saponin (cyan circles) and as a result of fusion with a 950H cell (green circles). Exponential fits (solid black lines) of the decaying green fluorescence and the obtained decay rates are shown. (B) Similar to panel A, but for the virus fusion with 800H cells in the absence (blue circles) or in the presence (open circles) of 0.1 mg/ml R99 peptide. (C) Half-times (T₅₀) of the NC-eGFP release in 950H (red bars), 950L (gray bars) and 800H (blue bars) cells. The T₅₀ values were determined from the exponential decay coefficients, as shown in panels A and B. The distribution of T₅₀ for saponin-formed lytic pores in single virions is shown by an open bar.